protein extraction for use in ELISA

James Munro via (by jmunro from
Wed Jan 2 14:42:26 EST 2008

I am hoping for some suggestions for a protein that I am trying to extract for detection in an ELISA.
I have a protein that is 22 kDa which is soluble in sodium borate at either a low pH (<4) or high pH (>9). In its pure form I can detects it in a ELISA when using specific antibodies. When the protein is in commercial fish feeds I can not longer detect it. 
Has any one got an extraction method that I could try? 

More information about the Methods mailing list