denaturing electrophoresis with paraformaldehyde?

Ed Siefker via methods%40net.bio.net (by ebs15242 from creighton.edu)
Thu Jan 10 16:45:58 EST 2008


Hi, I need to do some denaturing RNA agarose gels.  The protocol
says I need 37% formaldehyde, I have paraformaldehyde on hand in
the lab.  Can I make 37% formaldehyde out of this?  What's the
stoichiometry between PFA and formaldehyde?  Thanks lots
-Ed



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