RNA purification

yan sun via methods%40net.bio.net (by yansun2005 from gmail.com)
Thu Jan 10 20:05:10 EST 2008


Hi guys,
I am working on RNA purification using denatured 20% 19:1 acryl/bis gel.
before loading, total RNA is ~50 nmole, after running the gel and elution,
my target RNA only 5 nmole left. supposed the yield is 18 nmole. I done this
before. but I just can  not repeat the result as before.
I wonder if my RNA degrade already? any problem with Gel or elutor?
Thank you for your help,


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