high molecular plasmid DNA

WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Sat Jan 12 05:56:51 EST 2008

that means: make the alkaline lysis step in plasmid isolation as short
as possible! Some protocols state "5 min", but for standard lab
strains of E. coli that's complete overkill.

Add SDS/NaOH (lysis solution), mix shortly by snipping with your
fingers, put on ice, add neutralizing solution and mix again
immediately. Never vortex, as this would shear genomic DNA.

Best regards,


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