high molecular plasmid DNA

WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Sat Jan 12 05:56:51 EST 2008


that means: make the alkaline lysis step in plasmid isolation as short
as possible! Some protocols state "5 min", but for standard lab
strains of E. coli that's complete overkill.

Add SDS/NaOH (lysis solution), mix shortly by snipping with your
fingers, put on ice, add neutralizing solution and mix again
immediately. Never vortex, as this would shear genomic DNA.

Best regards,

Wo


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