Calculating Protein Concentration

StewJW via methods%40net.bio.net (by stewjw from gmail.com)
Fri Jan 18 04:02:34 EST 2008


On 17 Jan, 12:46, "Yvonne Couch" <yvonne.co... from dpag.ox.ac.uk> wrote:
> I have two proteins of different molecular weights, how do I calculate the
> molarity of any solution I dissolve them in?  For example Protein 1 is 15kDa
> and 1mg is dissolved in 1ml of water, how do I calculate how many moles this
> is?

so 1 mg / ml or 0.001 g / ml

0.001 / 15 000 = 66.66 nmol /ml (66.66 x 10^-9 mole / ml)

or 66.66 x 1000 = 66.66 µmol / L or 66.66 µM  (M = Molar = mol / L)



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