Transfecting IMR90 cells

Alain CUZANGE via methods%40net.bio.net (by acuzange from polyplus-transfection.com)
Mon Jan 21 13:57:28 EST 2008


Hi Tim,

As far as I know it is not a very difficult cell line. 
If you go to the Invitrogen link http://www.invitrogen.com/content.cfm?pageID=9559&fuseaction=CellLines.dsp_applicationDataDetails&applicationDataID=1601
you will find a brief protocol with LipoFectamin but definitely I recommend LipoFECTAMIN 2000 for those cells. 
Concerning CaPO4, it is a boring and not so reproductive method.
A good alternative is to use jetPEI (a polymer based product). The protocol is faster than LipoFectamine 2000 (you can transfect in presence of serum and antibiotics, no need to change the medium). JetPEI is also less toxic at confluency between 50 to 70%.

Good luck

Alain 

-----Message d'origine-----
De : Tim Eggleston [mailto:timegg113 from gmail.com] 
Envoyé : mardi 15 janvier 2008 21:32
À : Alain CUZANGE
Cc : methods from magpie.bio.indiana.edu
Objet : Re: Transfecting IMR90 cells

I would like to transfect DNA.  Thank you for your help.

Tim

On Jan 15, 2008 10:55 AM, Alain CUZANGE
<acuzange from polyplus-transfection.com> wrote:
> Hi Tim
> Would you like to transfect DNA or siRNA?
> Alain
> acuzange from polyplus-transfection.com
>
> -----Message d'origine-----
> De: methods-bounces from oat.bio.indiana.edu [mailto:methods-bounces from oat.bio.indiana.edu] De la part de Tim Eggleston
> Envoyé: mardi 15 janvier 2008 17:37
> À: methods from magpie.bio.indiana.edu
> Objet: Transfecting IMR90 cells
>
>
> I was wondering if anyone has attempted to transfect IMR90 fibroblast
> cells using a Lipofectamine or calcium phosphate transfection.  If so,
> how well did it work?  Thanks.
>
> Tim Eggleston
>
> --
> Tim Eggleston
> Horne Lab
> Research Assistant
> 2-505 Bowen Science Building
> University of Iowa
>
>
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-- 
Tim Eggleston
Horne Lab
Research Assistant
2-505 Bowen Science Building
University of Iowa



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