pcDNA 3.1/Myc-His(+) B cutting problems
yitingw from gmail.com
via methods%40net.bio.net
(by yitingw from gmail.com)
Mon Jan 28 22:07:47 EST 2008
I have some problem by using pcDNA 3.1/Myc-His(+) B.
I am trying to cut Hind3 and Kpn1cutting sites in order to insert my
favorite gene.
Since I have tried several times and still can not get my favorite
gene inserted, I was wondering
is it possible to cut pcDNA 3.1/Myc-His(+)B by Hind3 and Kpn1 at the
same time?
Would the two restriction enzymes compete with each other since their
cutting sits are near by?
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