pcDNA 3.1/Myc-His(+) B cutting problems

yitingw from gmail.com via methods%40net.bio.net (by yitingw from gmail.com)
Mon Jan 28 22:07:47 EST 2008


I have some problem by using pcDNA 3.1/Myc-His(+) B.

I am trying to cut Hind3 and Kpn1cutting sites in order to insert my
favorite gene.

Since I have tried several times and still can not get my favorite
gene inserted, I was wondering

is it possible to cut pcDNA 3.1/Myc-His(+)B by Hind3 and Kpn1 at the
same time?

Would the two restriction enzymes compete with each other since their
cutting sits are near by?



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