DNA EXTRACTION

Jayakumar, R via methods%40net.bio.net (by R.Jayakumar from roswellpark.org)
Tue Jan 29 16:04:21 EST 2008


I have worked with sesame plant DNA extraction. I can understand your
problem. I spent months trying to get intact DNA from OIlseeds like
gingelly (sesame).  SDS alone may not work with oilseed plants and
seeds.  What you could do is use a protocol like Dellaporte (search for
a paper in nucleic acidsresearch in 1991 or 1990).  protocols that use
N-Laurylsarcosine and PVP (to complex polyphenolic compounds) is best.
You can reduce the viscosity of the DNA sup after extraction with these
two compounds pretty well. 
Jay
  

-----Original Message-----
From: methods-bounces from oat.bio.indiana.edu
[mailto:methods-bounces from oat.bio.indiana.edu] On Behalf Of DK
Sent: Tuesday, January 29, 2008 9:03 AM
To: methods from magpie.bio.indiana.edu
Subject: Re: DNA EXTRACTION

In article <mailman.403.1201615132.2451.methods from net.bio.net>, "kamalaker
nasani" <agbiok4 from gmail.com> wrote:
>Hi good morning to everyone
>
>I have to extract DNA from seeds.  I am facing many problems due to fat

>content.  Anybody having protocol for extraction of DNA from high fat 
>content seed protein.

I would use 1% SDS in lysis buffer followed by two extractions with
chloroform. This will remove both SDS and fat/lipids.

DK
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