Methods Digest, Vol 32, Issue 29

kamalaker nasani via methods%40net.bio.net (by agbiok4 from gmail.com)
Thu Jan 31 22:37:19 EST 2008


Thanx a lot for reply for fat content dna extraction

On 1/31/08, methods-request from oat.bio.indiana.edu <
methods-request from oat.bio.indiana.edu> wrote:
>
> Send Methods mailing list submissions to
>        methods from net.bio.net
>
> To subscribe or unsubscribe via the World Wide Web, visit
>        http://www.bio.net/biomail/listinfo/methods
> or, via email, send a message with subject or body 'help' to
>        methods-request from net.bio.net
>
> You can reach the person managing the list at
>        methods-owner from net.bio.net
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Methods digest..."
>
>
> Today's Topics:
>
>   1. Re: reg DNA extraction from water (Aawara Chowdhury)
>   2. Re: reg DNA extraction from water (Pow Joshi)
>   3. Re: reg DNA extraction from water (Pow Joshi)
>   4. Re: reg DNA extraction from water (Christian Praetorius)
>   5. DNA extraction from water (aswini sekar)
>   6. DNA extraction from water (aswini sekar)
>   7. Increment of mRNA level after siRNA (Kamalian, Laleh)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 30 Jan 2008 11:55:06 GMT
> From: Aawara Chowdhury <aawara from pontiff-playground.org>
> Subject: Re: reg DNA extraction from water
> To: methods from net.bio.net
> Message-ID: <uAZnj.37656$Ft5.14032 from newsfe15.lga>
>
> In <mailman.416.1201655154.2451.methods from net.bio.net>,
> aswini sekar <aswinisekar16 from gmail.com> wrote:
>
> > hi i am Aswini,
> > I am doing my project in metagenomics.
> > I couldnt extract DNA from water.I dont know why?????//
> > Can anybody help me?////
> > Pl send me new protocol or the  general problems in DNA extraction from
> > water.
>
> There is no polywater, water has no memory, and it has no genetic
> material :-)
>
> AC
> --
> Email: echo 36434455860060025978157675027927670979097959886449930P | dc
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 29 Jan 2008 20:19:14 -0500
> From: "Pow Joshi" <pow.joshi from gmail.com>
> Subject: Re: reg DNA extraction from water
> To: "aswini sekar" <aswinisekar16 from gmail.com>
> Cc: Methods from magpie.bio.indiana.edu
> Message-ID:
>        <710764ea0801291719p4de6ad65p4c12e85ceda15e49 from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> On 29/01/2008, aswini sekar <aswinisekar16 from gmail.com> wrote:
> >
> > hi i am Aswini,
> > I am doing my project in metagenomics.
> > I couldnt extract DNA from water.I dont know why?????//
> > Can anybody help me?////
> > Pl send me new protocol or the  general problems in DNA extraction from
> > water.
> > Thank u
>
>
>
> hey Aswini,
>
> just a suggestion: you may want to send a coherent description of your
> problem, for eg: what is your starting material, what method of extraction
> you use, how you detect your DNA etc., and NOT something which is
> hurriedly
> sent of in a state of panic. Folks on this list will be able to help you
> better that way.
>
> best,
> Pow
>
> > _______________________________________________
> > Methods mailing list
> > Methods from net.bio.net
> > http://www.bio.net/biomail/listinfo/methods
> >
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 29 Jan 2008 20:20:38 -0500
> From: "Pow Joshi" <pow.joshi from gmail.com>
> Subject: Re: reg DNA extraction from water
> To: "aswini sekar" <aswinisekar16 from gmail.com>
> Cc: Methods from magpie.bio.indiana.edu
> Message-ID:
>        <710764ea0801291720w746ac69dqeba90cdf06e33eaa from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> On 29/01/2008, Pow Joshi <pow.joshi from gmail.com> wrote:
> >
> >
> >
> > On 29/01/2008, aswini sekar <aswinisekar16 from gmail.com> wrote:
> > >
> > > hi i am Aswini,
> > > I am doing my project in metagenomics.
> > > I couldnt extract DNA from water.I dont know why?????//
> > > Can anybody help me?////
> > > Pl send me new protocol or the  general problems in DNA extraction
> from
> > > water.
> > > Thank u
> >
> >
>
> ...also, if you want to look up very basic protocols, please refer to
> Current protocold in Molecular Biology or Molecular Biology, a Laboratory
> manual by Sambrook et al.
>
>
>
> hey Aswini,
> >
> > just a suggestion: you may want to send a coherent description of your
> > problem, for eg: what is your starting material, what method of
> extraction
> > you use, how you detect your DNA etc., and NOT something which is
> hurriedly
> > sent of in a state of panic. Folks on this list will be able to help you
> > better that way.
> >
> > best,
> > Pow
> >
> > > _______________________________________________
> > > Methods mailing list
> > > Methods from net.bio.net
> > > http://www.bio.net/biomail/listinfo/methods
> > >
> >
> >
>
>
> ------------------------------
>
> Message: 4
> Date: Wed, 30 Jan 2008 09:57:04 +0000
> From: Christian Praetorius <prae from gmx.net>
> Subject: Re: reg DNA extraction from water
> To: methods from net.bio.net
> Message-ID: <60avvaF1q1blsU1 from mid.individual.net>
> Content-Type: text/plain; charset=us-ascii
>
> "aswini sekar" <aswinisekar16 from gmail.com> wrote:
>
> >I couldnt extract DNA from water.I dont know why?????//
> >Can anybody help me?////
> >Pl send me new protocol or the  general problems in DNA extraction from
> >water.
>
> Help would be possible if you describe, what you did (and which
> protocol you used).
>
> Christian
>
> --
> X-no-Sig: yes
>
>
> ------------------------------
>
> Message: 5
> Date: Thu, 31 Jan 2008 05:25:03 +0530
> From: "aswini sekar" <aswinisekar16 from gmail.com>
> Subject: DNA extraction from water
> To: Methods from magpie.bio.indiana.edu
> Message-ID:
>        <95ccb3c10801301555l797ce7f4h741dc497964240b3 from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> hai i am Aswini,
>      i am doing my project in metagenomics.I need to isolate DNA from well
> water contaminated with Tannery effluents.
> Can any one suggest a simple protocol for DNA isolation from contaminated
> water???????????
>
>
> ------------------------------
>
> Message: 6
> Date: Thu, 31 Jan 2008 06:08:30 +0530
> From: "aswini sekar" <aswinisekar16 from gmail.com>
> Subject: DNA extraction from water
> To: Methods from magpie.bio.indiana.edu
> Message-ID:
>        <95ccb3c10801301638t3ada8197n3d3ca56ed7b1af62 from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> hai,
>    Metagenomics is the study of Unculturable microbes in the environment.
>   In this we use to Extract DNA directly from any environmental samples
> like water,soil,sediments,feces,urine,gut of insects,etc.
>   what does DNA extraction from water mean is to directly extract DNA from
> the mirobes present in the water without culturing.
> it is possible to extract DNA of all extremophiles in water.
> I think now u could understand better.
> now if u could find any protocols if possible send me
> Aswini
>
>
> ------------------------------
>
> Message: 7
> Date: Thu, 31 Jan 2008 14:24:48 -0000
> From: "Kamalian, Laleh" <L.Kamalian from liverpool.ac.uk>
> Subject: Increment of mRNA level after siRNA
> To: "methods" <methods from magpie.bio.indiana.edu>
> Message-ID:
>        <31DC2DFFD70C174B8E3072803F894F91046C59 from EVSSTAFF1.livad.liv.ac.uk>
> Content-Type: text/plain;       charset="iso-8859-1"
>
>
> Hi
> Thanks to people who helped me last time.  This time its not a desperate
> question.  Only to ask your opinions.  I have successfully used a plasmid
> vector to subside the mRNA level of the gene I'm working on.  I used 4
> different oligonucleotides to insert into the plasmid and used them to
> target my gene.  Although most of the plasmids have successfully decreased
> the mRNA level (different levels of course), one of the clones has actually
> increased the level of the gene's mRNA.  I can't explain it.  Some of my
> colleagues suggest the compensatory mechanisms of the cell.  I think its
> simply not being knocked down or maybe an error in real time PCR? I had seen
> this before working on another cell line.  What do you think?
> LK
>
>
>
> ------------------------------
>
> _______________________________________________
> Methods mailing list
> Methods from net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>
> End of Methods Digest, Vol 32, Issue 29
> ***************************************
>



-- 
URS
Kamalaker Nasani



"The illiterate of the 21st  Century will not be those
who cannot read or write.  The illiterate will be those
who cannot learn, unlearn  and relearn"
-George Bernard Shaw


More information about the Methods mailing list