RNA on denaturing gels
(by pjie2 from cam.ac.uk)
Wed Jul 9 05:54:04 EST 2008
On Tue, 8 Jul 2008, utsuxs from hotmail.com wrote:
> These are just random thoughts.
> I forgot where tRNA ends up but that could be the 3500nt band.
Transfer RNAs are ~75 bp, so.... no.
> And contamination. Your ingredients and equipment, including your gel
> box might contaminated with RNase which is hell to get rid of.
> Wouldn't matter if your RNA is RNA and intact, you drop it in an RNase
> contaminated environment, well so long RNA.
Yes, but the OP says his ssRNA ladder ran fine, which argues against
degradation in the gel.
To the OP - are you sure what size ribosomal bands you're expecting from
Paramecium? I strongly doubt it'll be the exact same pattern/size you'd
expect from mammalian cells.
More information about the Methods