Trypsin/EDTA

[Jose de las Heras]

"DK" <dk from no.email.thankstospam.net> wrote in message 
news:4Ahdk.2981$dc7.927 from newsfe06.lga...
>
> "You could just wash with EDTA and add little trypsin but the
> cost of doing it in sterile pipettes and time exceeds the cost of
> the crude trypsin preparation used in cell culture."
>
> DK

I normally just wash with a bit of PBS and then put a small amount of 
trypsin, like Kyle said, but that's because I usually deal with only a few 
flasks at any one time. When you are facing several stacks containing a 
hundred or so dishes, adding extra trypsin to dilute remaining medium and 
removing most of it is a much more palatable approach...

Jose