DNA crosslinking on nylon memberane

TC via methods%40net.bio.net (by tracyscience from gmail.com)
Wed Jul 16 15:39:55 EST 2008


Hello,

I am trying to crosslink DNA to nylon membrane for southern blots.  Besides
traditional UV crosslinking (may induce DNA damage) and baking (causes DNA
to denature), are there any other way to crosslink DNA onto nylon
memberanes?  For my purpose, I hope minimize DNA damage and keep that DNA in
native condition for the Southern blots.

THANKS~!

Tracy


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