DNA crosslinking on nylon memberane

Peter Ellis via methods%40net.bio.net (by pjie2 from cam.ac.uk)
Thu Jul 17 03:49:26 EST 2008

TC wrote:
> Hello,
> I am trying to crosslink DNA to nylon membrane for southern blots.  Besides
> traditional UV crosslinking (may induce DNA damage) and baking (causes DNA
> to denature), are there any other way to crosslink DNA onto nylon
> memberanes?  For my purpose, I hope minimize DNA damage and keep that DNA in
> native condition for the Southern blots.

Why do you want your DNA in native condition?  Unless you denature it, 
it won't be accessible for your probe to bid to and you will have 
reduced signal.


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