DNA crosslinking on nylon memberane

[Jose de las Heras]

"TC" <tracyscience from gmail.com> wrote in message 
news:mailman.777.1216254116.3533.methods from net.bio.net...
> Hello,
>
> I am trying to crosslink DNA to nylon membrane for southern blots. 
> Besides
> traditional UV crosslinking (may induce DNA damage) and baking (causes DNA
> to denature), are there any other way to crosslink DNA onto nylon
> memberanes?  For my purpose, I hope minimize DNA damage and keep that DNA 
> in
> native condition for the Southern blots.
>
> THANKS~!
>
> Tracy

Several positively charged membranes claimed to be ok for use without 
conventional crosslinking. Personally I always UV-crosslink out of habit.

I doubt the damage on immobilised DNA is going to give you any trouble, and 
I doubt immobilised DNA on a dry membrane is going to denature with heat... 
and besides, for successful hybridisation you want the DNA to be denatured.

You may try a positively charged membrane to bypass heating/UV.

Out of curiosity, what are you trying to do?

Jose