Proliferation assay in suspension cells

Dr Engelbert Buxbaum via methods%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Tue Jun 3 10:09:33 EST 2008


Am 30.05.2008, 07:21 Uhr, schrieb Kamalian, Laleh  
<L.Kamalian from liverpool.ac.uk>:

> Hi and thanks to everyone who have been helping me so far. I am in  
> trouble again. I need a protocol for proliferation assay in cells that  
> grow in suspension.  The method that is used in our lab is MTT assay  
> which is as I underestand for adherent cells.

You can also use it for suspension cells, the only problem is that you  
have to replace the medium by the MTT solution and the MTT solution by the  
propanol/HCl reagent. That is a lot easier with anchored cells where you  
simply flick of the solution to be discarded. With suspended cells you'll  
have to spin, which is more work. But any other assay you might use  
suffers from the same problem.


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