(by novalidaddress from nurfuerspam.de)
Wed Jun 4 11:14:05 EST 2008
Just had a glance at the patent: Genial things usually are simple: The
main principle of RNA later seems to be guess what:
Ammonium sulfate precipitation!
Just precipitate all the bad RNAses, then they won't bother you. Of
course, some more goodies are added: eg EDTA
100% saturation is 541.8g/l solution @ 25degC, so 80g/100ml should not
be feasible without special tricks. Or does it mean: Add 80g to 100ml.
That would make a total volume of about 150 ml, this just might work.
Further reading on ammonium sulfate prec. may be found here:
More information about the Methods