RNA Later-Crystal!!

WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Wed Jun 4 11:14:05 EST 2008


Just had a glance at the patent: Genial things usually are simple: The
main principle of RNA later seems to be guess what:

Ammonium sulfate precipitation!

Just precipitate all the bad RNAses, then they won't bother you. Of
course, some more goodies are added: eg EDTA

100% saturation is 541.8g/l solution @ 25degC, so 80g/100ml should not
be feasible without special tricks. Or does it mean: Add 80g to 100ml.
That would make a total volume of about 150 ml, this just might work.

Further reading on ammonium sulfate prec. may be found here:

http://www.encorbio.com/protocols/AM-SO4.htm

Enjoy!
Wo


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