Methods Digest, Vol 37, Issue 8

Virash Gupta via methods%40net.bio.net (by virashkgupta from gmail.com)
Wed Jun 11 11:15:08 EST 2008


For LB-Ampicillin use only Sodium Salt. Others will not be soluble in water


On 6/10/08, methods-request from oat.bio.indiana.edu
<methods-request from oat.bio.indiana.edu> wrote:
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> Today's Topics:
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>   1. Ampicillin (Wen Huang)
>   2. Inactivation of DNase I (Gouttesoulard Marina - Saint-Beauzire )
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> ----------------------------------------------------------------------
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> Message: 1
> Date: Mon, 09 Jun 2008 14:45:19 -0500
> From: Wen Huang <huang_wen from ustc.edu>
> Subject: Ampicillin
> To: Methods from magpie.bio.indiana.edu
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> Hi,
>
> I was wondering what kind of ampicillin should I use to make LB-amp+
> media and LB-agar plates
>
> I searched the internet and there are three versions of amplicillins
>
> 1. ampicillin
> 2. ampicillin sodium salt
> 3. ampicillin trihydrate
>
> I am pretty sure people use ampicillin sodium salt for cell culture,
> just want to make sure that what I should use for bacteria culture.
>
> By the way, when these things go into water, do they have the same
> activity?
>
> Thanks,
> Wen
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 10 Jun 2008 15:30:13 +0200
> From: "Gouttesoulard Marina - Saint-Beauzire "
>        <marina.gouttesoulard from lonza.com>
> Subject: Inactivation of DNase I
> To: <methods from magpie.bio.indiana.edu>
> Message-ID:
>        <2751B10B7FB0A849860E80F52E796B490362A41F from chvex24.lonzagroup.net>
> Content-Type: text/plain;       charset="iso-8859-1"
>
> Hello,
> Have you solved your inactivation DNase problem?
> I have also a problem in inactivation of DNase. I inactive DNase by heating step 10min at 75°C. To check if the inactivation is efficient, I add 1ng DNA in my inactivated sample and I compare the results obtained in PCR with a control containing 1 ng DNA. There is a shift of 2 cycles with the inactivated sample. So I think that the Dnase is not totally inactivated and act on DNA added to destroy it. Have you got some solution to inactivate Dnase without damage RNA.
> Thanks a lot.
> rq : the Dnase that I used was the Turbo Dnase from ambion and I already test the inactivator of ambion's kit.
>
>
> Marina
> > mailto:marina.gouttesoulard from lonza.com
> > http://www.lonza.com
> >
> >
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-- 
Dr V K Gupta
Sr Microbiologist (Molecular Biology)
Insect Molecular Biology Lab
Department of Entomology
Punjab Agricultural University
Ludhiana (Pb)-141004- India
M: 09815963210



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