Methods Digest, Vol 37, Issue 8

Virash Gupta via methods%40net.bio.net (by virashkgupta from gmail.com)
Wed Jun 11 11:15:54 EST 2008


For DNase inactivation use Heating in combination with added EDTA


On 6/11/08, Virash Gupta <virashkgupta from gmail.com> wrote:
> For LB-Ampicillin use only Sodium Salt. Others will not be soluble in water
>
>
> On 6/10/08, methods-request from oat.bio.indiana.edu
> <methods-request from oat.bio.indiana.edu> wrote:
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> >   1. Ampicillin (Wen Huang)
> >   2. Inactivation of DNase I (Gouttesoulard Marina - Saint-Beauzire )
> >
> >
> > ----------------------------------------------------------------------
> >
> > Message: 1
> > Date: Mon, 09 Jun 2008 14:45:19 -0500
> > From: Wen Huang <huang_wen from ustc.edu>
> > Subject: Ampicillin
> > To: Methods from magpie.bio.indiana.edu
> > Message-ID: <8BDEEB82-30A2-4B48-A871-51FE0FE33F43 from ustc.edu>
> > Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
> >
> > Hi,
> >
> > I was wondering what kind of ampicillin should I use to make LB-amp+
> > media and LB-agar plates
> >
> > I searched the internet and there are three versions of amplicillins
> >
> > 1. ampicillin
> > 2. ampicillin sodium salt
> > 3. ampicillin trihydrate
> >
> > I am pretty sure people use ampicillin sodium salt for cell culture,
> > just want to make sure that what I should use for bacteria culture.
> >
> > By the way, when these things go into water, do they have the same
> > activity?
> >
> > Thanks,
> > Wen
> >
> >
> >
> > ------------------------------
> >
> > Message: 2
> > Date: Tue, 10 Jun 2008 15:30:13 +0200
> > From: "Gouttesoulard Marina - Saint-Beauzire "
> >        <marina.gouttesoulard from lonza.com>
> > Subject: Inactivation of DNase I
> > To: <methods from magpie.bio.indiana.edu>
> > Message-ID:
> >        <2751B10B7FB0A849860E80F52E796B490362A41F from chvex24.lonzagroup.net>
> > Content-Type: text/plain;       charset="iso-8859-1"
> >
> > Hello,
> > Have you solved your inactivation DNase problem?
> > I have also a problem in inactivation of DNase. I inactive DNase by heating step 10min at 75°C. To check if the inactivation is efficient, I add 1ng DNA in my inactivated sample and I compare the results obtained in PCR with a control containing 1 ng DNA. There is a shift of 2 cycles with the inactivated sample. So I think that the Dnase is not totally inactivated and act on DNA added to destroy it. Have you got some solution to inactivate Dnase without damage RNA.
> > Thanks a lot.
> > rq : the Dnase that I used was the Turbo Dnase from ambion and I already test the inactivator of ambion's kit.
> >
> >
> > Marina
> > > mailto:marina.gouttesoulard from lonza.com
> > > http://www.lonza.com
> > >
> > >
> >
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> > End of Methods Digest, Vol 37, Issue 8
> > **************************************
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>
>
> --
> Dr V K Gupta
> Sr Microbiologist (Molecular Biology)
> Insect Molecular Biology Lab
> Department of Entomology
> Punjab Agricultural University
> Ludhiana (Pb)-141004- India
> M: 09815963210
>


-- 
Dr V K Gupta
Sr Microbiologist (Molecular Biology)
Insect Molecular Biology Lab
Department of Entomology
Punjab Agricultural University
Ludhiana (Pb)-141004- India
M: 09815963210



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