Methods Digest, Vol 37, Issue 10

Virash Gupta via methods%40net.bio.net (by virashkgupta from gmail.com)
Fri Jun 13 02:17:38 EST 2008


concentarting Cry1Ac from Bt-plant is not easy as it is truncated
protein. better and easy to produce recombinant synthetic cry1Ac
protein using cloned genes available from dr Altosaar or others and
cRy1Ac specific antibodies.


On 6/12/08, methods-request from oat.bio.indiana.edu
<methods-request from oat.bio.indiana.edu> wrote:
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> Today's Topics:
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>   1. Re: Protein work (AK)
>   2. Re: Methods Digest, Vol 37, Issue 8 (Virash Gupta)
>   3. Re: Methods Digest, Vol 37, Issue 8 (Virash Gupta)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 11 Jun 2008 09:54:26 -0500
> From: "AK" <akhan357 from sbcglobal.net>
> Subject: Re: Protein work
> To: methods from net.bio.net
> Message-ID: <pGR3k.11441$Ri.1369 from flpi146.ffdc.sbc.com>
>
> how about using ultrafiltration devices sold by amicon (part of millipore
> now). 10 fold concentration is easily achieved by these. one example is here
> http://www.millipore.com/catalogue/item/42423
> AK
>
> "kamalaker nasani" <agbiok4 from gmail.com> wrote in message
> news:mailman.405.1213190547.3533.methods from net.bio.net...
> > ---------- Forwarded message ----------
> > From: kamalaker nasani <agbiok4 from gmail.com>
> > Date: Wed, Jun 11, 2008 at 6:20 PM
> > Subject: Protein work
> > To: methods from net.bio.net, Plant Tissue Culture <PLANT-TC from lists.umn.edu>
> >
> >
> > Good  morning,
> >
> >
> > I need a protocol to enrich or to concentrate protein of specific KD  from
> > plant material.
> >
> > Details:  I need to concentrate 10 times more of trasgene protein from
> > seed
> > powder.  The gene is Cry1Ac.
> >
> > If anybody having any material related to this please share with me.
> >
> >
> >
> > --
> > URS
> > Kamalaker Nasani
> >
> >
> >
> > "The illiterate of the 21st Century will not be those
> > who cannot read or write. The illiterate will be those
> > who cannot learn, unlearn and relearn"
> > -George Bernard Shaw
> >
> >
> >
> > --
> > URS
> > Kamalaker Nasani
> >
> >
> >
> > "The illiterate of the 21st Century will not be those
> > who cannot read or write. The illiterate will be those
> > who cannot learn, unlearn and relearn"
> > -George Bernard Shaw
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 11 Jun 2008 21:45:08 +0530
> From: "Virash Gupta" <virashkgupta from gmail.com>
> Subject: Re: Methods Digest, Vol 37, Issue 8
> To: methods from oat.bio.indiana.edu
> Message-ID:
>        <f5d8ce8c0806110915gc17b881t309063e72f1e79a1 from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> For LB-Ampicillin use only Sodium Salt. Others will not be soluble in water
>
>
> On 6/10/08, methods-request from oat.bio.indiana.edu
> <methods-request from oat.bio.indiana.edu> wrote:
> > Send Methods mailing list submissions to
> >        methods from net.bio.net
> >
> > To subscribe or unsubscribe via the World Wide Web, visit
> >        http://www.bio.net/biomail/listinfo/methods
> > or, via email, send a message with subject or body 'help' to
> >        methods-request from net.bio.net
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> >        methods-owner from net.bio.net
> >
> > When replying, please edit your Subject line so it is more specific
> > than "Re: Contents of Methods digest..."
> >
> >
> > Today's Topics:
> >
> >   1. Ampicillin (Wen Huang)
> >   2. Inactivation of DNase I (Gouttesoulard Marina - Saint-Beauzire )
> >
> >
> > ----------------------------------------------------------------------
> >
> > Message: 1
> > Date: Mon, 09 Jun 2008 14:45:19 -0500
> > From: Wen Huang <huang_wen from ustc.edu>
> > Subject: Ampicillin
> > To: Methods from magpie.bio.indiana.edu
> > Message-ID: <8BDEEB82-30A2-4B48-A871-51FE0FE33F43 from ustc.edu>
> > Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
> >
> > Hi,
> >
> > I was wondering what kind of ampicillin should I use to make LB-amp+
> > media and LB-agar plates
> >
> > I searched the internet and there are three versions of amplicillins
> >
> > 1. ampicillin
> > 2. ampicillin sodium salt
> > 3. ampicillin trihydrate
> >
> > I am pretty sure people use ampicillin sodium salt for cell culture,
> > just want to make sure that what I should use for bacteria culture.
> >
> > By the way, when these things go into water, do they have the same
> > activity?
> >
> > Thanks,
> > Wen
> >
> >
> >
> > ------------------------------
> >
> > Message: 2
> > Date: Tue, 10 Jun 2008 15:30:13 +0200
> > From: "Gouttesoulard Marina - Saint-Beauzire "
> >        <marina.gouttesoulard from lonza.com>
> > Subject: Inactivation of DNase I
> > To: <methods from magpie.bio.indiana.edu>
> > Message-ID:
> >        <2751B10B7FB0A849860E80F52E796B490362A41F from chvex24.lonzagroup.net>
> > Content-Type: text/plain;       charset="iso-8859-1"
> >
> > Hello,
> > Have you solved your inactivation DNase problem?
> > I have also a problem in inactivation of DNase. I inactive DNase by heating step 10min at 75°C. To check if the inactivation is efficient, I add 1ng DNA in my inactivated sample and I compare the results obtained in PCR with a control containing 1 ng DNA. There is a shift of 2 cycles with the inactivated sample. So I think that the Dnase is not totally inactivated and act on DNA added to destroy it. Have you got some solution to inactivate Dnase without damage RNA.
> > Thanks a lot.
> > rq : the Dnase that I used was the Turbo Dnase from ambion and I already test the inactivator of ambion's kit.
> >
> >
> > Marina
> > > mailto:marina.gouttesoulard from lonza.com
> > > http://www.lonza.com
> > >
> > >
> >
> > This communication and its attachments, if any, may contain confidential and privileged information the use of
> > which by other persons or entities than the intended recipient is prohibited. If you receive this transmission
> > in error, please contact the sender immediately and delete the material from your system.
> >
> >
> > ------------------------------
> >
> > _______________________________________________
> > Methods mailing list
> > Methods from net.bio.net
> > http://www.bio.net/biomail/listinfo/methods
> >
> > End of Methods Digest, Vol 37, Issue 8
> > **************************************
> >
>
>
> --
> Dr V K Gupta
> Sr Microbiologist (Molecular Biology)
> Insect Molecular Biology Lab
> Department of Entomology
> Punjab Agricultural University
> Ludhiana (Pb)-141004- India
> M: 09815963210
>
>
>
> ------------------------------
>
> Message: 3
> Date: Wed, 11 Jun 2008 21:45:54 +0530
> From: "Virash Gupta" <virashkgupta from gmail.com>
> Subject: Re: Methods Digest, Vol 37, Issue 8
> To: methods from oat.bio.indiana.edu
> Message-ID:
>        <f5d8ce8c0806110915y7bc9cd45xbadcab3b3b448559 from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> For DNase inactivation use Heating in combination with added EDTA
>
>
> On 6/11/08, Virash Gupta <virashkgupta from gmail.com> wrote:
> > For LB-Ampicillin use only Sodium Salt. Others will not be soluble in water
> >
> >
> > On 6/10/08, methods-request from oat.bio.indiana.edu
> > <methods-request from oat.bio.indiana.edu> wrote:
> > > Send Methods mailing list submissions to
> > >        methods from net.bio.net
> > >
> > > To subscribe or unsubscribe via the World Wide Web, visit
> > >        http://www.bio.net/biomail/listinfo/methods
> > > or, via email, send a message with subject or body 'help' to
> > >        methods-request from net.bio.net
> > >
> > > You can reach the person managing the list at
> > >        methods-owner from net.bio.net
> > >
> > > When replying, please edit your Subject line so it is more specific
> > > than "Re: Contents of Methods digest..."
> > >
> > >
> > > Today's Topics:
> > >
> > >   1. Ampicillin (Wen Huang)
> > >   2. Inactivation of DNase I (Gouttesoulard Marina - Saint-Beauzire )
> > >
> > >
> > > ----------------------------------------------------------------------
> > >
> > > Message: 1
> > > Date: Mon, 09 Jun 2008 14:45:19 -0500
> > > From: Wen Huang <huang_wen from ustc.edu>
> > > Subject: Ampicillin
> > > To: Methods from magpie.bio.indiana.edu
> > > Message-ID: <8BDEEB82-30A2-4B48-A871-51FE0FE33F43 from ustc.edu>
> > > Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
> > >
> > > Hi,
> > >
> > > I was wondering what kind of ampicillin should I use to make LB-amp+
> > > media and LB-agar plates
> > >
> > > I searched the internet and there are three versions of amplicillins
> > >
> > > 1. ampicillin
> > > 2. ampicillin sodium salt
> > > 3. ampicillin trihydrate
> > >
> > > I am pretty sure people use ampicillin sodium salt for cell culture,
> > > just want to make sure that what I should use for bacteria culture.
> > >
> > > By the way, when these things go into water, do they have the same
> > > activity?
> > >
> > > Thanks,
> > > Wen
> > >
> > >
> > >
> > > ------------------------------
> > >
> > > Message: 2
> > > Date: Tue, 10 Jun 2008 15:30:13 +0200
> > > From: "Gouttesoulard Marina - Saint-Beauzire "
> > >        <marina.gouttesoulard from lonza.com>
> > > Subject: Inactivation of DNase I
> > > To: <methods from magpie.bio.indiana.edu>
> > > Message-ID:
> > >        <2751B10B7FB0A849860E80F52E796B490362A41F from chvex24.lonzagroup.net>
> > > Content-Type: text/plain;       charset="iso-8859-1"
> > >
> > > Hello,
> > > Have you solved your inactivation DNase problem?
> > > I have also a problem in inactivation of DNase. I inactive DNase by heating step 10min at 75°C. To check if the inactivation is efficient, I add 1ng DNA in my inactivated sample and I compare the results obtained in PCR with a control containing 1 ng DNA. There is a shift of 2 cycles with the inactivated sample. So I think that the Dnase is not totally inactivated and act on DNA added to destroy it. Have you got some solution to inactivate Dnase without damage RNA.
> > > Thanks a lot.
> > > rq : the Dnase that I used was the Turbo Dnase from ambion and I already test the inactivator of ambion's kit.
> > >
> > >
> > > Marina
> > > > mailto:marina.gouttesoulard from lonza.com
> > > > http://www.lonza.com
> > > >
> > > >
> > >
> > > This communication and its attachments, if any, may contain confidential and privileged information the use of
> > > which by other persons or entities than the intended recipient is prohibited. If you receive this transmission
> > > in error, please contact the sender immediately and delete the material from your system.
> > >
> > >
> > > ------------------------------
> > >
> > > _______________________________________________
> > > Methods mailing list
> > > Methods from net.bio.net
> > > http://www.bio.net/biomail/listinfo/methods
> > >
> > > End of Methods Digest, Vol 37, Issue 8
> > > **************************************
> > >
> >
> >
> > --
> > Dr V K Gupta
> > Sr Microbiologist (Molecular Biology)
> > Insect Molecular Biology Lab
> > Department of Entomology
> > Punjab Agricultural University
> > Ludhiana (Pb)-141004- India
> > M: 09815963210
> >
>
>
> --
> Dr V K Gupta
> Sr Microbiologist (Molecular Biology)
> Insect Molecular Biology Lab
> Department of Entomology
> Punjab Agricultural University
> Ludhiana (Pb)-141004- India
> M: 09815963210
>
>
>
> ------------------------------
>
> _______________________________________________
> Methods mailing list
> Methods from net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>
> End of Methods Digest, Vol 37, Issue 10
> ***************************************
>


-- 
Dr V K Gupta
Sr Microbiologist (Molecular Biology)
Insect Molecular Biology Lab
Department of Entomology
Punjab Agricultural University
Ludhiana (Pb)-141004- India
M: 09815963210



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