protocol for membrane receptor isolation

Behdani via (by behdani from
Mon Jun 23 09:03:39 EST 2008

Hello All
I have to isolate cytoplasmic membrane receptor from 293 cell line (Human Kidney) to immunize rabbit for polyclonal antibody production. I am looking for a protocol (lysis buffer and other steps) which contains reagents which save native form of membrane proteins. Could somebody help me?
Thank you
Mahdi Behdani
Ph.D Student

More information about the Methods mailing list