Inactivation of DNase I

domo via (by rimask from
Tue Jun 24 03:24:59 EST 2008

80C for 20 min

"Gouttesoulard Marina - Saint-Beauzire " <marina.gouttesoulard from> 
wrote in message news:mailman.385.1213106791.3533.methods from
Have you solved your inactivation DNase problem?
I have also a problem in inactivation of DNase. I inactive DNase by heating 
step 10min at 75°C. To check if the inactivation is efficient, I add 1ng DNA 
in my inactivated sample and I compare the results obtained in PCR with a 
control containing 1 ng DNA. There is a shift of 2 cycles with the 
inactivated sample. So I think that the Dnase is not totally inactivated and 
act on DNA added to destroy it. Have you got some solution to inactivate 
Dnase without damage RNA.
Thanks a lot.
rq : the Dnase that I used was the Turbo Dnase from ambion and I already 
test the inactivator of ambion's kit.

> mailto:marina.gouttesoulard from

This communication and its attachments, if any, may contain confidential and 
privileged information the use of
which by other persons or entities than the intended recipient is 
prohibited. If you receive this transmission
in error, please contact the sender immediately and delete the material from 
your system. 

More information about the Methods mailing list