T4 PNK

Dr. Hiranya S. Roychowdhury via methods%40net.bio.net (by hroychow from nmsu.edu)
Mon Jun 23 15:41:58 EST 2008


Good question!  I think long time ago ... in the last century ... Maniatis
et al.'s Lab Manual described the specificity of these enzymes.  I have
not really used dNTP for phosphoryltion, so I don't know how efficient it
would be.  Structurally the two are similar enough.


> Hi all,
>
> have kinda silly question: Can T4PNK use dATP instead of rATP to
> phosphorilate DNA?
> Thanks ,
> Peter
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-- 
Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Health & Public Services
Dona Ana Community College
New Mexico State University
Las Cruces, NM 88003



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