(by peter.ianakiev from gmail.com)
Mon Jun 23 21:26:55 EST 2008
On Jun 23, 9:58 pm, d... from no.email.thankstospam.net (DK) wrote:
> In article <mailman.543.1214259080.3533.meth... from net.bio.net>, hroyc... from nmsu.edu wrote:
> >Good question! I think long time ago ... in the last century ... Maniatis
> >et al.'s Lab Manual described the specificity of these enzymes. I have
> >not really used dNTP for phosphoryltion, so I don't know how efficient it
> >would be. Structurally the two are similar enough.
> I've never heard of any kinase being able to use dATP with any
> reasonable efficiency. I'd think that evolution worked out
> a way for things being pretty good at distinguishing oxy vs
> and desoxy ribose. Much like DNAse vs RNAse.
I thought so too, but it seems that DNA end repair by T4DNA pol +
T4PNK works fine just with dNTP (not supplemented with rATP). Strange
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