T4 PNK

peter via methods%40net.bio.net (by peter.ianakiev from gmail.com)
Mon Jun 23 21:26:55 EST 2008


On Jun 23, 9:58 pm, d... from no.email.thankstospam.net (DK) wrote:
> In article <mailman.543.1214259080.3533.meth... from net.bio.net>, hroyc... from nmsu.edu wrote:
> >Good question!  I think long time ago ... in the last century ... Maniatis
> >et al.'s Lab Manual described the specificity of these enzymes.  I have
> >not really used dNTP for phosphoryltion, so I don't know how efficient it
> >would be.  Structurally the two are similar enough.
>
> I've never heard of any kinase being able to use dATP with any
> reasonable efficiency. I'd think that evolution worked out
> a way for things being  pretty good at distinguishing oxy vs
> and desoxy ribose. Much like DNAse vs RNAse.
>
> DK

I thought so too, but it seems that DNA end repair by T4DNA pol +
T4PNK works fine just with dNTP (not supplemented with rATP). Strange
Peter


More information about the Methods mailing list