Lab generics Wiki? (Was: Re: Western Stripping Buffer?)
(by ucgatan from ucl.ac.uk)
Thu Jun 26 12:09:03 EST 2008
On Thu, 26 Jun 2008, Tom Anderson wrote:
> On Thu, 26 Jun 2008, DK wrote:
> > In article <b161ec7b-b0e5-43ab-bfc7-8feb40b202ba from m36g2000hse.googlegroups.com>, WS <novalidaddress from nurfuerspam.de> wrote:
> > >On Jun 25, 9:58 pm, "Heather Woodward" <heather.woodw... from gmail.com>
> > >wrote:
> > >> To strip westerns, I use Re-Blot Mild Antibody Stripping Solution (Chemicon
> > >> #2502). It's extremely simple and effective, especially when re-probing for
> > >> phospho-proteins.
> > >
> > >You may have that cheaper: just use 10mM or 100mM NaOH. (mild or
> > >strong variant). It's not really a secret - just read the included
> > >MSDS *^_^*
> > For many years now, I've had the idea of starting a web site that would
> > list compositions of companies' proprietary reagents and suggest cheap
> > generic alternatives. Simple things like Quik Solution (DMSO), Mild
> > Antibody Elution Buffer (3.6 M MgCl2) and such. It does not even have to
> > be precise - educated guesses, supported by evidence and references
> > would also be very useful (such as Qiagen's secret buffer compositions,
> > a subject of many posts here over the years) .
> > These days, Wiki format would seem to be the most logical and convenient
> > way to go. But I am too clueless on how to host a web page and too lazy
> > to learn.
> > Anyone wants to start one???
> Ask and ye shall receive:
> You have to register, but it's no huge deal. Now, get in there and reveal
> some secrets!
Okay, edit: you have to register, then apply to join the wiki, then i have
to approve you, which is clearly complete rubbish. I've sent a
pre-approved invite to DK, but this isn't going to scale. I'll look for a
non-totally-missing-the-point wiki supplier tomorrow ...
Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
(t) +44 (20) 76797264 (f) +44 (20) 76797805 (e) thomas.anderson from ucl.ac.uk
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