Promoter charecterization query - Reg.

prashant singam via methods%40net.bio.net (by prashantsingam from gmail.com)
Mon Jun 30 04:01:54 EST 2008


Dr. Chai,
             I have isolated a promoter of a lignin biosynthetic pathway
gene. I would like to characterize the same in a plant system. In this
regard i have a query : should I clone the 5' UTR region  with translation
start site of the gene along with upstream region containing promoter
elements or not?
I would appreciate if you help me inthis regard at the earliest.
Thanking you,
S.Prashant
Research Scholar
c/o Prof.P.B.Kavi Kishor
Dept.of Genetics
Osmania University
Hyderabad.


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