Albumin depletion from sheep plasma

[Arianmanesh, Mitra]

Dear Pow
 
Actually, I am doing Albumin depletion as I need the plasma for running
the 2-D gel. if there is Albumin in the plasma, the gained 2-D gel would
be very fussy and smeary due to lots of loaded Albumin as it covers
other proteins on the 2-D gel. Now I'm trying Zoom fractionator for
separating Albumin based on its PH, however it was not successful so
far. I would be happy if you advise me.
 
Sincerely,
 
Mitra
 

________________________________

From: Pow Joshi [mailto:pow.joshi from gmail.com] 
Sent: 04 March 2008 00:05
To: Arianmanesh, Mitra
Cc: methods from magpie.bio.indiana.edu
Subject: Re: Albumin depletion from sheep plasma




On 03/03/2008, Arianmanesh, Mitra <r06ma6 from abdn.ac.uk> wrote: 

	Dears
	
	I need to deplete Albumin from sheep plasma while there is no
defined
	and perfect method for it. Actually, I used several methods for
that
	such as Immunoprecipitation and SwellGel Blue Albumin Removal
Kit but I
	could not get good results. So, now I need to know that how to
deplete
	Abumin from the sheep plasma. Can anybody give me an advise to
do that



yes, that is a tough one.. Perhaps, you could just use Blue sepharose
CL-6B (trade name I think is Cibacron), and run your plasma one it as a
column a few times or perhaps do a serial culk incubation. 
Now, if you wish to purify antibodies, you could use the afffinity
columns; if you don't care about having or not having antibodies in the
plasma/serum, you could probably use an ion exchanger as well.
...actually, I think I am a bit fuzzy here, and can be able to help you
better if you let us know what exactly you wish to get out of the
plasma.

best
Pow



	well?
	
	Kind regards,
	
	Mitra
	
	
	
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The University of Aberdeen is a charity registered in Scotland, No SC013683.