Triton X-100

[Kyle Legate]

diana_barbosa from sapo.pt wrote:
> Hello,
> 
> I'm having some trouble with the solubilization of proteins contained in 
> a lipidic secretion we're studying (using 2DE).
> To overcome this problem, I'm preparing a series of trials with 
> different lysis buffers, prepared with detergents 
> alternative/complementary to CHAPS.
> One of the alternatives mentioned in the literature is Triton X-100, but 
> in some papers it appears that this detergent doesn't help solubilize 
> hydrophobic proteins but rather precipitates them, creating a 2 
> fractions (hydrophilic and hydrophobic proteins). Is this true or am I 
> misinterpreting?
> 
> Any help will be very welcome.
> Thank you,
> 
> Diana
> 
I think you mean Triton X-114, which is used for cloud point assays.

http://www.ncbi.nlm.nih.gov/pubmed/6257680?ordinalpos=5&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum