homemade ECL

[Aawara Chowdhury]

In <63egs3F270vvmU1 from mid.individual.net>,
 Jose de las Heras <josenet from tiscali.co.uk> wrote:

> I don't do a lot of westerns, but others in my lab do and noone complained 
> about sensitivity when we stopped buying the commercial version. 

The Pierce Supersignal reagents that Dima referred to are far more sensitive
than Amersham's ECL (ECL plus) reagents.  We also use a home-made ECL 
reagent that is similar to yours, and the sensitivity of our reagent is 
similar to Amersham's ECL reagent (or Pierce's ECL reagent).

The Supersignal reagents permit detection of 10 - 50 femtograms of protein.

In practical terms this means that if we use a particular antibody at a
1:1000 dilution with our home-made ECL reagent, we can use it at 1:15000
with the Supersignal Femto reagent.  Useful for some Abs that are very
expensive.

AC
-- 
Email: echo 36434455860060025978157675027927670979097959886449930P | dc