Triton X-100

[Dr Engelbert Buxbaum]

Am 07.03.2008, 13:39 Uhr, schrieb Kyle Legate <legatek from hotmail.com>:


> Insolubility in TX-100 is one of the hallmarks of so-called "lipid  
> rafts", and very well might be the cause of clogged columns, but if the  
> lipidic secretion the OP is working with is low in cholesterol, TX-100  
> should definitely be considered in the screen. If the secretion is  
> cholesterol rich, adding some digitonin might help to solubilize it.

I agree. One may also consider Tween detergents (properties similar to  
Triton) or the Elugents, which are sugar-alkane based industrial  
detergents (much cheaper than OG or DM).