Dr Engelbert Buxbaum
(by engelbert_buxbaum from hotmail.com)
Mon Mar 10 08:52:30 EST 2008
Am 07.03.2008, 13:39 Uhr, schrieb Kyle Legate <legatek from hotmail.com>:
> Insolubility in TX-100 is one of the hallmarks of so-called "lipid
> rafts", and very well might be the cause of clogged columns, but if the
> lipidic secretion the OP is working with is low in cholesterol, TX-100
> should definitely be considered in the screen. If the secretion is
> cholesterol rich, adding some digitonin might help to solubilize it.
I agree. One may also consider Tween detergents (properties similar to
Triton) or the Elugents, which are sugar-alkane based industrial
detergents (much cheaper than OG or DM).
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