Dam and Dcm methylation problem

Emad via methods%40net.bio.net (by e_barouki from hotmail.com)
Fri Mar 14 08:19:52 EST 2008


 

 

Hello 

Can somebody tell me if I can use the XL1 or NEB5 alpha E coli for cloning
plasmids which will be used for digestion by enzymes that are sensitive to
Dam, Dcm and CG methylation. I know that these strains are not Dcm- ,Dam-
strains. However can I demethylate my isolated plasmid later on, for further
enzymatic digestion and how?

Thank in Advance

Emad

 



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