DMSO in PCR

[chovek69]

On Mar 18, 1:03 am, d... from no.email.thankstospam.net (DK) wrote:
> In article <mailman.1120.1205773333.2451.meth... from net.bio.net>, "popi Arapini" <karap... from gmail.com> wrote:
>
> >hI! everybody,
> >I'am using DMSO to standardize my PCR (high GC content)
> >I've read in a protocol that, once DMSO in the thermowell tube, it shall
> >not  be spinned  down . Does anybody  know why??
>
> No reason. You heard wrong.

Yes this is nonsense. 5-10% DMSO disolves perfectly in PCR buffer ....
so no bother. In our lab 5% DMSO seems best for many assays.