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[Sudheendra Rao N R]

I use the following transfer buffer.
for 500ml
350ml MilliQ Water
50ml 10X Electrode Buffer (Tris.Glycine-pH 8.3)
100ml Methanol

Per blot 50-80ml is sufficient (semi Dry transfer). I dont re-use the
transfer buffer..instead i keep used ones to take out ponceau staining after
its done.

Sudheendra.

On Wed, Mar 19, 2008 at 4:29 PM, Siddhartha Parida <sidharth4 from gmail.com>
wrote:

> What is composition of electro transfer buffer for transferring DNA to
> nitrocellulose membranes
> by electroblotting  ?
>
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