"Triton"s

Dr Engelbert Buxbaum via methods%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Fri Mar 21 12:42:10 EST 2008


Am 16.03.2008, 08:35 Uhr, schrieb kalva <siheng.xiang from gmail.com>:

> when extract proteins from membrane, usually we try Triton X100 first,
> but there are different kinds of Tritons in crystallization kits, with
> different different numbers in their names. what's the differences?
> where can i find their structures? and what does the number mean?

The average length of the ethylenglycol chain is different, resulting in  
different properties (cmc, \bar{m}, Kraft point). Remember that Tritons  
are industrial detergents, that is wild mixtures of related compounds. For  
crystallisation I'd prefer detergents with defined structure, e.g. C12E8  
or the like.

Some detergent properties you can get from  
http://psyche.uthct.edu/shaun/SBlack/detergnt.html, also from the web  
pages of manufacturers like Anatrace.


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