Annealing temp using Quikchange SDM kit

Clarisa Bejar via methods%40net.bio.net (by cbejar from berkeley.edu)
Thu Mar 20 13:47:54 EST 2008

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Hi,

 

I’m designing my Quikchange-SDM and have a question regarding the annealing
temperature. What is the best way to determine the annealing temperature
since, in this case, primers should be designed with a minimum Tm= 78ºC? (so
the annealing temperature= Tm- 5ºC wouldn’t apply here)

Thanks for your feedback!

 

Clarisa

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