soft agar transformation assay

AS via methods%40net.bio.net (by Antonio.Sarikas from gmail.com)
Mon Mar 31 18:03:03 EST 2008

Previous message: GST contamination of GST fusion protein
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

Hi, could anyone recommend a protocol for a soft agar transfromation
assays for mouse embryonic fibroblasts and human diploid fibroblasts?

Some technical questions:

1. which agarose do you use (DNA grade?)
2. how do you get the agarase sterile?
3. which medium do you use: 2x DMEM?

Your advice is greatly appreciated - thanks!

AS

Previous message: GST contamination of GST fusion protein
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

More information about the Methods mailing list