Salmon sperm DNA and Northern again

Barbara MacGregor via methods%40net.bio.net (by bmacgreg from unc.edu)
Fri May 2 13:05:05 EST 2008


Hi,

We had that happen once, and in the end the only thing that helped  
was switching membrane manufacturers. The company swore they hadn't  
changed anything, but neither had we, so.

Maybe you can get free samples from a couple of different companies?

Barbara MacGregor

On May 2, 2008, at 1:06 PM, methods-request from oat.bio.indiana.edu wrote:

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>    1. salmon sperm DNA and Northern again (shiri shiri)
>
> From: shiri  shiri <shirisau from hotmail.com>
> Date: May 1, 2008 1:40:27 PM EDT
> To: <methods from magpie.bio.indiana.edu>
> Subject: salmon sperm DNA and Northern again
>
>
> Hello,
>
> You posted once on a problem you had with your northern hybridization.
> As you posted, I experience the same problem, unspecific binding  
> all over
> the membrane, even in a blank one. I changed all my compounds,  
> increased the
> concentration of the blocking solution but nothing works...
> The weird thing it worked great for many years but now no one in  
> our lab,
> even ones who were previously successful in it, cannot solve this  
> issue...
>
> I was wondering if you had a chance to solve this problem????
>
> I would appreciate any help/suggestion
>
> THANKS
> SHIRI
>
>
>
>
>
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