co-transfecting with multiple vectors

Wendy Grus via methods%40net.bio.net (by wgrus from umich.edu)
Mon May 5 13:19:41 EST 2008


Hi all-
I am transfecting some HEK 293 T cells to transiently express a few
different genes.  Some of these genes are on pcDNA3.1 vectors while some are
on pCI vectors.  Will having them on different vectors affect their
expression levels?  Will I have to play with the concentrations of each?
 Should I put all the genes in one vector (singly)?

Thanks,
Wendy Grus


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