co-transfecting with multiple vectors

Sudheendra Rao N R via methods%40net.bio.net (by sudhee26 from gmail.com)
Thu May 8 09:49:05 EST 2008


Hey, look in to some of these
http://www.springerlink.com/content/u3531825942t18v5/

http://www.bio.net/hypermail/methds-reagnts/2001-September/090500.html

http://www.bio.net/hypermail/methds-reagnts/2001-September/090499.html

best,
sudheendra.

On Mon, May 5, 2008 at 11:49 PM, Wendy Grus <wgrus from umich.edu> wrote:

> Hi all-
> I am transfecting some HEK 293 T cells to transiently express a few
> different genes.  Some of these genes are on pcDNA3.1 vectors while some
> are
> on pCI vectors.  Will having them on different vectors affect their
> expression levels?  Will I have to play with the concentrations of each?
>  Should I put all the genes in one vector (singly)?
>
> Thanks,
> Wendy Grus
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