co-transfecting with multiple vectors

Sudheendra Rao N R via (by sudhee26 from
Thu May 8 09:49:05 EST 2008

Hey, look in to some of these


On Mon, May 5, 2008 at 11:49 PM, Wendy Grus <wgrus from> wrote:

> Hi all-
> I am transfecting some HEK 293 T cells to transiently express a few
> different genes.  Some of these genes are on pcDNA3.1 vectors while some
> are
> on pCI vectors.  Will having them on different vectors affect their
> expression levels?  Will I have to play with the concentrations of each?
>  Should I put all the genes in one vector (singly)?
> Thanks,
> Wendy Grus
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