Methods Digest, Vol 36, Issue 4

Jim Young via methods%40net.bio.net (by jyyoungjimmy from gmail.com)
Sat May 10 16:38:43 EST 2008


That is simple. Our lab is using this pre-cast gel product, No EB, with
prestain DNA marker. About $40 for 10 gels and ready to use, very nice.
http://www.genscript.com/agarose_gel_kits.html


On Mon, May 5, 2008 at 1:05 PM, <methods-request from oat.bio.indiana.edu> wrote:

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> Today's Topics:
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>   1. methylene blue in the DNA sample (Gita Bangera)
>   2. Re: methylene blue in the DNA sample (Maximilian Haeussler)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sun, 4 May 2008 13:04:48 -0700
> From: "Gita Bangera" <gbangera from bcc.ctc.edu>
> Subject: methylene blue in the DNA sample
> To: <methods from magpie.bio.indiana.edu>
> Message-ID:
>        <ED7D39393F6D1742941173A4D35944BE0CF1BD87 from EAGLE.bcc.ctc.edu>
> Content-Type: text/plain;       charset="us-ascii"
>
> Hello,
>
>
>
> Has anyone tried adding methylene blue to their DNA sample before
> running it on the gel? Since methylene blue stains DNA, I am wondering
> if that would work.  I am not interested in cutting out the bands from
> this gel or using the DNA in any way. I just want my students to be able
> to visualize the DNA on the gel and I really don't want to have to deal
> with EtBr.
>
>
>
> Thank you for your time.
>
>
>
> Gita
>
>
>
> Gita Bangera Ph.D.
>
> Instructor, Life Sciences (Biology)
>
> Bellevue Community College
>
> Tel: 425-564-4031
>
> Fax: 425-564-4125
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 5 May 2008 11:44:30 +0200
> From: "Maximilian Haeussler" <maximilianh from gmail.com>
> Subject: Re: methylene blue in the DNA sample
> To: "Gita Bangera" <gbangera from bcc.ctc.edu>
> Cc: methods from magpie.bio.indiana.edu
> Message-ID:
>        <76f031ae0805050244g73dd3f4bk86a23d9a7f85081c from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> nick from biteisizebio wrote about these "safe" stains recently, also
> calculating cost per 100ml:
> http://bitesizebio.com/2008/03/03/ethidium-bromide-the-alternatives/
>
> he writes that methylene blue is "Post strain only, in 0.025% (w/v)
> methylene blue in water." and according to him only bands above 500ng
> are visible.
>
> methylene blue is also part of those "run your DNA at home" instructions:
>
> http://www.scq.ubc.ca/the-macgyver-project-genomic-dna-extraction-and-gel-electrophoresis-experiments-using-everyday-materials/
>
> cheers
> Max
>
> 2008/5/4 Gita Bangera <gbangera from bcc.ctc.edu>:
> > Hello,
> >
> >
> >
> > Has anyone tried adding methylene blue to their DNA sample before
> > running it on the gel? Since methylene blue stains DNA, I am wondering
> > if that would work.  I am not interested in cutting out the bands from
> > this gel or using the DNA in any way. I just want my students to be able
> > to visualize the DNA on the gel and I really don't want to have to deal
> > with EtBr.
> >
> >
> >
> > Thank you for your time.
> >
> >
> >
> > Gita
> >
> >
> >
> > Gita Bangera Ph.D.
> >
> > Instructor, Life Sciences (Biology)
> >
> > Bellevue Community College
> >
> > Tel: 425-564-4031
> >
> > Fax: 425-564-4125
> >
> >
> >
> > _______________________________________________
> > Methods mailing list
> > Methods from net.bio.net
> > http://www.bio.net/biomail/listinfo/methods
> >
>
>
>
> --
> Maximilian Haeussler,
> tel: +33 6 12 82 76 16
>
>
>
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> End of Methods Digest, Vol 36, Issue 4
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