Methods Digest, Vol 36, Issue 3

Jim Young via methods%40net.bio.net (by jyyoungjimmy from gmail.com)
Sat May 10 16:32:11 EST 2008


About the DNA purification problem, you may use glycogen to purify your DNA.
You may google glycogen for details. It is so easy. By the way, I used the
products and services from http://www.genscript.com/. Their PCR products are
not expensive and pretty good.


On Sat, May 3, 2008 at 1:05 PM, <methods-request from oat.bio.indiana.edu> wrote:

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> Today's Topics:
>
>   1. Re: Salmon sperm DNA and Northern again (Barbara MacGregor)
>   2. EmulsionPCR (peter)
>   3. ethanol precipitation (yan sun)
>   4. Cb5R Assay (Sarah)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 2 May 2008 14:05:05 -0400
> From: Barbara MacGregor <bmacgreg from unc.edu>
> Subject: Re: Salmon sperm DNA and Northern again
> To: methods from oat.bio.indiana.edu
> Message-ID: <15F271AA-1F1A-4592-8B7E-005EAC67F7AF from unc.edu>
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> Hi,
>
> We had that happen once, and in the end the only thing that helped
> was switching membrane manufacturers. The company swore they hadn't
> changed anything, but neither had we, so.
>
> Maybe you can get free samples from a couple of different companies?
>
> Barbara MacGregor
>
> On May 2, 2008, at 1:06 PM, methods-request from oat.bio.indiana.edu wrote:
>
> > Send Methods mailing list submissions to
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> > When replying, please edit your Subject line so it is more specific
> > than "Re: Contents of Methods digest..."
> > Today's Topics:
> >
> >    1. salmon sperm DNA and Northern again (shiri shiri)
> >
> > From: shiri  shiri <shirisau from hotmail.com>
> > Date: May 1, 2008 1:40:27 PM EDT
> > To: <methods from magpie.bio.indiana.edu>
> > Subject: salmon sperm DNA and Northern again
> >
> >
> > Hello,
> >
> > You posted once on a problem you had with your northern hybridization.
> > As you posted, I experience the same problem, unspecific binding
> > all over
> > the membrane, even in a blank one. I changed all my compounds,
> > increased the
> > concentration of the blocking solution but nothing works...
> > The weird thing it worked great for many years but now no one in
> > our lab,
> > even ones who were previously successful in it, cannot solve this
> > issue...
> >
> > I was wondering if you had a chance to solve this problem????
> >
> > I would appreciate any help/suggestion
> >
> > THANKS
> > SHIRI
> >
> >
> >
> >
> >
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>
> ------------------------------
>
> Message: 2
> Date: Fri, 2 May 2008 13:31:14 -0700 (PDT)
> From: peter <peter.ianakiev from gmail.com>
> Subject: EmulsionPCR
> To: methods from net.bio.net
> Message-ID:
>        <f0a4ef96-db5f-4e2f-ae0f-39978de842a6 from c65g2000hsa.googlegroups.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi group, I need to make emulsion in the emulsionPCR uniform. Any
> suggestions?
> Thanks,
> Peter
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 2 May 2008 14:50:48 -0500
> From: "yan sun" <yansun2005 from gmail.com>
> Subject: ethanol precipitation
> To: Methods from magpie.bio.indiana.edu
> Message-ID:
>        <a3be9a9a0805021250o5720286bqbc0fb00690f611e9 from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Dear all,
> I did spin label DNA experiment.
> I mixed my modified DNA with spin label material in DMF and fomamide
> solution. I get 100% label efficiency. (the analytical gel shows that there
> is only one spin  label DNA band).
> I was wondering how to purify my spin labeled DNA?  My other site spin
> labeled DNAs are not 100% spin label efficiency, so I RUN  big PAGE gel,
> and
> use elutor to purified my target spin labeled DNA. during this process, I
> lose quite amount material. that is why I don't want to run big gel this
> time.
> I made a research. looks like ethanol precipitation works.
> anyone have experience, please feel free to share.
> option 2. can I use freezing dry method?
>
> Thx a lot!!!
>
>
> --
> Y. F.S.
>
>
> ------------------------------
>
> Message: 4
> Date: Sat, 3 May 2008 15:47:48 +0300
> From: Sarah <hyrax3 from gmail.com>
> Subject: Cb5R Assay
> To: methods from magpie.bio.indiana.edu
> Message-ID:
>        <e6c69ec0805030547g30416e10k1aa234b26f0b716a from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hello,
>
>  I am looking for an assay to determine the activity of cytochrome b5
> reductase in blood. Can anyone help?
>
> Thank you.
>
> Sarah
>
>
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> End of Methods Digest, Vol 36, Issue 3
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