Blunt End Concatemerization

Ozan Aygun via (by metugenetics from
Wed May 14 16:26:27 EST 2008

Dear all,

I have annealed two complementary 20nt oligos to give
a blunt end fragment and would like to concatemerize
it to give a tandem copies of >15 fragments. I have
tried Roche T4 ligase without any success with
standard buffer conditions in a 50ul volume. 

Do you have any alternative suggestions?

Many thanks,



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