Blunt End Concatemerization

Ozan Aygun via methods%40net.bio.net (by metugenetics from yahoo.com)
Wed May 14 16:26:27 EST 2008


Dear all,

I have annealed two complementary 20nt oligos to give
a blunt end fragment and would like to concatemerize
it to give a tandem copies of >15 fragments. I have
tried Roche T4 ligase without any success with
standard buffer conditions in a 50ul volume. 

Do you have any alternative suggestions?

Many thanks,

Ozan


      



More information about the Methods mailing list