Blunt End Concatemerization

Jose de las Heras via methods%40net.bio.net (by josenet from tiscali.co.uk)
Wed May 14 20:23:41 EST 2008


"Ozan Aygun" <metugenetics from yahoo.com> wrote in message 
news:mailman.90.1210805268.3533.methods from net.bio.net...
> Dear all,
>
> I have annealed two complementary 20nt oligos to give
> a blunt end fragment and would like to concatemerize
> it to give a tandem copies of >15 fragments. I have
> tried Roche T4 ligase without any success with
> standard buffer conditions in a 50ul volume.
>
> Do you have any alternative suggestions?
>
> Many thanks,
>
> Ozan

Are the oligos phosphorylated?
usually you will get dephosphorylated oligos, unless you ask specifically, 
and these won't ligate.

Jose 




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