In situ hybridization with DNA probe
(by sonali_mookerjee from yahoo.com)
Mon May 19 15:26:35 EST 2008
I am planning to use in situ hybridization to detect gene expression patterns in plant tissue. Most of the protocols I have found use RNA probes although they acknowledge that using RNA probes is more difficult. Does anyone have a protocol for using DNA probes with DIG labelling? Or can anybody point to any publication that describes the technique?
Michigan State University
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