In situ hybridization with DNA probe

SONALI MOOKERJEE via methods%40net.bio.net (by sonali_mookerjee from yahoo.com)
Mon May 19 15:26:35 EST 2008


I am planning to use in situ hybridization to detect gene expression patterns in plant tissue.  Most of the protocols I have found use RNA probes although they acknowledge that using RNA probes is more difficult.  Does anyone have a protocol for using DNA probes with DIG labelling?  Or can anybody point to any publication that describes the technique?

Thanks
Sonali Mookerjee
Michigan State University



      


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