Methods Digest, Vol 36, Issue 16

Jim Young via methods%40net.bio.net (by jyyoungjimmy from gmail.com)
Mon May 19 20:38:22 EST 2008


Hi,

If you want to go further for IP-Western, a product will be very helpful to
you. The kit is optimized to heavy chains, light chains contamination. What
more excited! The kit cut the Western time to one hour.
See here: http://www.genscript.com/ip_western_tech.html

On Tue, May 20, 2008 at 1:04 AM, <methods-request from oat.bio.indiana.edu>
wrote:

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>   1. IP Protocols and hints (Bean Long)
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> Message: 1
> Date: Mon, 19 May 2008 11:24:40 +1000
> From: Bean Long <ben.long from yourfinger.anu.edu.au>
> Subject: IP Protocols and hints
> To: methods from net.bio.net
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> Hi all,
>
> I've recently got my hands on an antibody to a small protein that's part
> of an enormous complex (over 100 nm in diameter) and thought I might try
> some IP to purify it. I've also got some Protein G-sepharose beads in
> the fridge which I could use for IP. Can anyone offer protocols or point
> me in the direction of some to help me out? I'll need to do this under
> native conditions. I've done very little IP before, so any hints would
> be handy.
>
> Cheers,
>
> Bean
>
>
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