Dyeing proteins BEFORE SDS-PAGE

Dr Engelbert Buxbaum via methods%40net.bio.net (by engelbert_buxbaum from hotmail.com)
Wed May 21 10:28:17 EST 2008


Am 11.05.2008, 15:10 Uhr, schrieb Yoram Gerchman  
<gerchman from research.haifa.ac.il>:

>
> Greetings netters
> Does anyone know of a suitable protocol for dyeing proteins BEFORE  
> SDS-PAGE
> electrophoresis? I want to detect the proteins on a surface by dyeing,  
> scrap
> them and run the gel. I can re-dye the gel with Coomassie in the end.
> Many thanks
> Yoram

That should be possible with non-covalently binding dyes like amidoblack,  
coomassie or Ponceau, provided the surface does not bind them. You may  
have to play a little with the dilution.


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