IP Protocols and hints
(by akhan357 from sbcglobal.net)
Thu May 22 15:46:28 EST 2008
I recently succeeded in endogenous co-IP of some erythrocyte
membrane/peripheral proteins. the protocols and recipes in paper below may
provide you with beginning at least. as you know IP is trial and error with
different buffers/ detergents/ etc. However, you mention that you want to
purify?? this protein by IP? IMHO you may end up wasting a lot of time for
unusable amount of protein.
J Biol Chem. 2008 May 23;283(21):14600-9. Epub 2008 Mar 17.
Dematin and Adducin Provide a Novel Link between the Spectrin Cytoskeleton
and Human Erythrocyte Membrane by Directly Interacting with Glucose
"Bean Long" <ben.long from yourfinger.anu.edu.au> wrote in message
news:4830d6d8$1 from clarion.carno.net.au...
> Hi all,
> I've recently got my hands on an antibody to a small protein that's part
> of an enormous complex (over 100 nm in diameter) and thought I might try
> some IP to purify it. I've also got some Protein G-sepharose beads in the
> fridge which I could use for IP. Can anyone offer protocols or point me in
> the direction of some to help me out? I'll need to do this under native
> conditions. I've done very little IP before, so any hints would be handy.
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