IP Protocols and hints

Nick Theodorakis via methods%40net.bio.net (by nick.theodorakis from gmail.com)
Fri May 23 08:45:51 EST 2008


On May 23, 2:44 am, Bean Long <ben.l... from yourfinger.anu.edu.au> wrote:


>
> Many thanks AK. I s'pose 'enrich' would be a better word? The hope is to
> do a bit of proteomic work and subunit relative abundance analysis on
> it. Enough to run relative pure stuff on a gel would be handy. I had a
> little play with the Protein G-sepharsoe I found but ended up with
> bucket loads of what I assume is Protein G on my gels. I also think it's
> a little too old for successful IP, so I'm getting a new batch!

More likely it was IgG you saw on your gel. The heavy chains are
between 50-60 kDa and the light chains are 20-something-ish kDa.

Nick

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Nick Theodorakis
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