3 questions

[Nick Theodorakis]

On May 23, 6:17 am, "Kamalian, Laleh" <L.Kamal... from liverpool.ac.uk>
wrote:
> I have 3 questions if someone could help me with those please.
> 1. Could 2 different bands (size wise) be detected for the same protein using poly colonal and monoclonal antibodies?

Possibly, if there is proteolysis, processing, or alternative
splicing.


> 3.  In siRNA , I have knock down my gene of interest at the mRNA level (70-80% according to quantitative PCR x2 experiments), but western blot of the same colony cell protein lysates show no or less ( only 30% ) decrement.  Any explanation please?

The protein might have a long half-life. Or there are two or more
isoforms but the siRNA targets only one of them.

Nick

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Nick Theodorakis
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