Hydroshear DNA

peter via methods%40net.bio.net (by peter.ianakiev from gmail.com)
Tue May 27 05:21:10 EST 2008


On May 26, 2:44 pm, "Jose de las Heras" <jose... from tiscali.co.uk> wrote:
> "peter" <peter.ianak... from gmail.com> wrote in message
>
> news:5aa1d425-dcae-4ab1-8a6a-4b3d6a0a7e7b from k13g2000hse.googlegroups.com...
>
> > Hello guys,
> > I need some expertise in random DNA fragmentation. I was thinking of
> > hydroshearing DNA by passing trough syringe. Does anyone know the
> > diameter of the needle needed to shear the DNA? is there better
> > methods? - any experience is greatly appreciated.
> > Thanks,
> > Peter
>
> We used to "autoclave" (actually using a standard kitchen pressure cooker)
> wheat genomic DNA to fragment it. This was many years ago in another life
> and I have never tried that again. I can't remember the size range we
> obtained... but if you don't have a sonicator available (which would be the
> best option) it should be a simple thing to try and see if it suits you. I
> suspect you may find most of it is above the 0.5-2kb range you wish... but I
> just don't remember.
>
> Jose

Boiling (denaturing) is not an option, sorry. I need to clone, make
libraries.
-Peter


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