can i use EDTA instead of TEMED for SDS PAGE ?

Christian Praetorius via methods%40net.bio.net (by prae from gmx.net)
Wed May 28 05:20:15 EST 2008


surajit03 from yahoo.co.in wrote:

>Can  I use EDTA  instead of  TEMED for SDS PAGE ? if not why?

No, you can not. TEMED catalyzes the polymerisation, so your gel
wouldn't polymerize without it. http://en.wikipedia.org/wiki/TEMED

Christian

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